Growth Factors and Cytokines for Organoid and 3D Culture Systems
Novoprotein
Differentiation of iPSCs to Lung Organoids
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Figure 4. iPSC-derived lung organoids were cultured with Novoprotein cytokines and growth factors as specified in the table.
iPSC-derived Human Intestinal Organoid
Mouse Intestinal Organoid
Mouse Colonic Organoid
Mouse Gastric Organoid
Mouse Bile Duct Organoid
Mouse Pancreatic Organoid
Mouse Airway Organoid
Uncompromised Biological Activity
Each production lot undergoes rigorous ED50 potency testing to confirm bioactivity within our defined specifications to ensure optimal performance in sensitive 3D systems.
Human R-Spondin 1(Cat. No.: CX83)
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1-10ng/ml (QC verified).
Human Noggin (Cat. No.: CB89)
Measured by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 10-100ng/mL in the presence of 200 ng/mL of Recombinant Human BMP‑4(Cat. No.: CR93). (QC verified)
Human Wnt-3a V3(Cat. No.: C22R)
Measured by its ability to induce Topflash reporter activity in 293FT human embryonic kidney cells.The ED50 for this effect is 5-25 ng/mL (QC verified).
Human Activin A(Cat. No.: C687)
Measured by its ability to induce SMAD signaling in 293-Activin A Res cells(Cat. No.: XCC11). The ED50 for this effect is 1-5 ng/mL (QC verified).
Human FGF basic(Cat. No.: C046)
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Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.42 ng/ml (Regularly tested).
Human EGF(Cat. No.: C029)
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml (QC verified).
Consistency You Can Trust
High-performance cytokines and growth factors manufactured under our stringent QC standards to reduce variability, preserve signaling fidelity, and maintain culture stability across passages.
Human R-Spondin 1(Cat. No.: CX83)
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Figure 4. iPSC-derived lung organoids were cultured with Novoprotein cytokines and growth factors as specified in the table.
| Cytokines/GFs | Cat. No. |
| Activin A | C687 |
| BMP-4 | CR93 |
| FGF basic | C046 |
| FGF-10 | CR11 |
| FGF-7/KGF | CH73 |
| Noggin | CB89 |
| Vitronectin | C395 |
iPSC-derived Human Intestinal Organoid
| Cytokines/GFs | Cat. No. |
| Activin A | C687 |
| BMP-4 | CR93 |
| FGF-4 | CR08 |
| Wnt-3a | C22R |
| Noggin | CB89 |
| R-Spondin 1 | CX83 |
| EGF | C029 |
Mouse Intestinal Organoid
| Cytokines/GFs | Cat. No. |
| EGF | C029 |
| R-Spondin 1 | CX83 |
| Noggin | C028 |
Mouse Colonic Organoid
| Cytokines/GFs | Cat. No. |
| EGF | C029 |
| R-Spondin 1 | CX83 |
| Wnt-3a | C22R |
| Noggin | C028 |
Mouse Gastric Organoid
| Cytokines/GFs | Cat. No. |
| EGF | C029 |
| R-Spondin 1 | CX83 |
| Wnt-3a | C22R |
| Noggin | C028 |
| FGF-10 | CR11 |
Mouse Bile Duct Organoid
| Cytokines/GFs | Cat. No. |
| EGF | C029 |
| R-Spondin 1 | CX83 |
| FGF-10 | CR11 |
| HGF | CJ72 |
Mouse Pancreatic Organoid
| Cytokines/GFs | Cat. No. |
| EGF | C029 |
| R-Spondin 1 | CX83 |
| FGF-10 | CR11 |
| Noggin | C028 |
| FGF basic | C044 |
| VEGF164 | CX73 |
Mouse Airway Organoid
| Cytokines/GFs | Cat. No. |
| FGF-7/KGF | CH73 |
| R-Spondin 1 | CX83 |
| FGF-10 | CR11 |
| Noggin | C028 |
Uncompromised Biological Activity
Each production lot undergoes rigorous ED50 potency testing to confirm bioactivity within our defined specifications to ensure optimal performance in sensitive 3D systems.
Human R-Spondin 1(Cat. No.: CX83)
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1-10ng/ml (QC verified).
Human Noggin (Cat. No.: CB89)
Measured by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 10-100ng/mL in the presence of 200 ng/mL of Recombinant Human BMP‑4(Cat. No.: CR93). (QC verified)
Human Wnt-3a V3(Cat. No.: C22R)
Measured by its ability to induce Topflash reporter activity in 293FT human embryonic kidney cells.The ED50 for this effect is 5-25 ng/mL (QC verified).
Human Activin A(Cat. No.: C687)
Measured by its ability to induce SMAD signaling in 293-Activin A Res cells(Cat. No.: XCC11). The ED50 for this effect is 1-5 ng/mL (QC verified).
Human FGF basic(Cat. No.: C046)
.Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.42 ng/ml (Regularly tested).
Human EGF(Cat. No.: C029)
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml (QC verified).
Consistency You Can Trust
High-performance cytokines and growth factors manufactured under our stringent QC standards to reduce variability, preserve signaling fidelity, and maintain culture stability across passages.
Human R-Spondin 1(Cat. No.: CX83)
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