Cytokine

#C001, Recombinant Human EPO (C-6His)

#C001, Recombinant Human EPO (C-6His)

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
詳細介紹

#C001, Recombinant Human EPO (C-6His)
Recombinant Human Erythropoietin is produced by our Mammalian expression system and the target gene encoding Ala28-Arg193 is expressed with a 6His tag at the C-terminus. 

  • Accession: P01588
  • Known As: Erythropoietin; Epoetin; EPO
  • Mol Mass: 19.2 KDa
  • APMol Mass: 30-40 kDa, reducing conditions
  • Formulation Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
  • Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
  • Purity:

 

Greater than 95% as determined by reducing SDS-PAGE.

  • Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

  • Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

  • Shipping

The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.

  • Background
Interleukin-1 alpha (IL1α) is a cytokine member of the interleukin-1 family. IL-1 consists of two distinct forms: IL1α and IL1β that recognize the same cell surface receptors but are distinct proteins with approximately 25% amino acid sequence identity. IL1α is constitutively produced by epithelial cells and plays an essential role in maintenance of skin barrier function. Upon stimulation, a wide variety of cells including osteoblasts, monocytes, macrophages can be induced to express IL1α. IL1α possesses a wide range of metabolic, physiological, haematopoietic activities, and is critically involved in the regulation of the immune responses and inflammatory responses.

For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.